FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26373455
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG308690.11704571888666085No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG371500.010.34442980-81
AAAAGGG348450.010.31221578-79
CGAGAAT52100.09.4794931
CGGGGAT82750.08.8951491
AAAGGGG341100.08.3836280-81
AGGGGGG667300.08.19003382-83
CGGAAAT56300.08.1818751
CGGGAAT66650.08.0513331
GGGAAAT216950.07.94577931
CCCGTAT31850.07.90234471
CGCGAAT11553.506466E-87.81198021
CGAAAAT53100.07.5123241
AAAAAAG731700.07.50401776-77
TAGGGGG134150.07.45385150-51
CCGGGAT78650.07.42670061
CTCGGAT72600.07.39147951
CCGGAAT53250.07.2236211
CTCGAAT53250.07.2236211
CGGGATC75050.07.21091652
GCCGGAT67950.07.19842241
Produced by FastQC (version 0.11.5)