FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24730875
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG137200.012.75856850-51
AAAGGGG349800.012.74456780-81
CGCGATT9300.011.744671
AAGGGGG368500.010.899343582-83
AAAAGGG356650.010.65512180-81
AAAAAGG493750.010.31736478-79
TACCGTA17550.010.2817149
CGGGGAT72000.09.6957371
AGGGGGG624250.09.04028682-83
TAAAAAA816800.09.02055670-71
TATAGGG66100.08.65978748-49
CTCGAAT51850.08.3346731
GGGGGAA369850.08.27637720-21
CGGGAAT56450.08.0761291
CGGAAAT51000.08.0080031
CGGGCAT86450.07.96524141
GGGGGGA615150.07.682189520-21
GCGGAAT38650.07.61795041
CCCGTAT27500.07.5982911
CGCGAAT11352.1663982E-77.5313461
Produced by FastQC (version 0.11.5)