FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25310191
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG406300.013.29317680-81
TAGGGGG150200.013.172150-51
AAGGGGG431500.012.49523282-83
AAAGGGG402650.011.74434180-81
AAAAAGG549550.010.23347578-79
TAAAAAA867100.010.0500970-71
AAAAAAG813950.010.0429678-79
CGCGAAT10951.8189894E-129.9751581
GGGAAAA368950.09.59712522-23
GGGGGAA429900.09.56218520-21
TATAGGG68600.09.10584448-49
CGGGAAT61750.08.9212781
GGGGAAT175300.08.83206762-63
CGGGGAT77650.08.8069821
CCCGTAT29900.08.2592041
GCGTCGT228450.08.22268794-95
GGGAATA129200.08.01339964-65
CGAGAAT49000.07.8504581
GGGGAAA395750.07.843062422-23
CGGGCAT89650.07.8400251
Produced by FastQC (version 0.11.5)