FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24446193
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG372100.012.77218880-81
TAGGGGG141600.012.37883950-51
AAGGGGG399800.011.97678782-83
AAAGGGG370650.011.143290580-81
GGGAAAA347600.09.87194822-23
AAAAAGG509050.09.54549978-79
AAAAAAG757200.09.50981578-79
TAAAAAA805600.09.47334670-71
GGGGGAA403000.09.38107920-21
CGCGTAT8853.2299795E-89.1225961
CGGGGAT72900.08.7295131
CGAGAAT50050.08.5398681
CGGGGTT82800.08.4314011
AAATCGT44850.08.2567134
CGGAAAT54300.08.2213061
CCGGGAT68400.08.1234871
GGGGAAT165900.08.11597762-63
TATAGGG60950.07.91075648-49
AATCGTG42100.07.8941955
CGGGAAT55450.07.793861
Produced by FastQC (version 0.11.5)