FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26462388
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG404100.013.99421780-81
AAGGGGG407350.013.48047382-83
TAGGGGG135450.011.69580850-51
GGGAAAA388800.011.04288222-23
TATAGGG70150.011.03763948-49
CGCGAAT9701.8189894E-1210.77090551
AAAAAAG823550.010.58323478-79
AAAGGGG391600.010.43797480-81
GGGGGAA440350.09.93356720-21
TAAAAAA917300.09.42991170-71
GGGGAAT183250.08.928349562-63
GCGGAAT41200.08.8755411
AAAAAGG561350.08.75747678-79
TAAGGGG116650.08.5312550-51
CGGGGAT79050.08.5307611
CGGAAAT61000.08.2523441
GGGGGGA675900.08.20002560-61
CCGGAAT55500.08.1289021
ATAAAAA671950.08.12065368-69
CTCGGAT70350.08.1006231
Produced by FastQC (version 0.11.5)