FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29721562
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG452550.012.48562880-81
TAGGGGG173800.012.25804350-51
AAGGGGG485400.011.68995382-83
AAAGGGG456350.010.87233680-81
CGGGAAT70900.09.9802831
AAAAAGG627350.09.57393378-79
GGGAAAA421550.09.52607722-23
GGGGGAA483000.09.5186420-21
TAAAAAA1016300.09.50225970-71
AAAAAAG941650.09.21574578-79
CCGGGAT84300.08.9572021
TATAGGG76300.08.84066848-49
CGCTAAT14650.08.7524361
GGGGAAT205900.08.63824862-63
CGAATCG19650.08.45587353
CGCGTAT11554.0345185E-98.2233891
CGCGAAT13902.910383E-118.1997241
CGGGGAT90300.08.0464911
GGGGAAA449450.07.693068522-23
CTCGGAT82200.07.68391661
Produced by FastQC (version 0.11.5)