FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23820144
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG378200.012.28399880-81
AAGGGGG414900.011.23770182-83
TAGGGGG127150.010.98406150-51
AAAGGGG375200.010.67934680-81
AAAAAAG755550.09.43323178-79
TAAAAAA804150.09.38990370-71
AAAAAGG516600.09.18541578-79
GGGGGAA397350.09.10219520-21
GGGAAAA334900.09.03386822-23
CGGGGTT83900.08.66031551
CGACGAT28650.08.4537581
GCGGTAT27100.08.4115531
CCGGGAT67050.08.2868851
CGGGGAT72550.07.92049261
CGGGAAC86700.07.887651
GCGTCGT197750.07.865939694-95
CGGACGT24800.07.85120731
CGGGAAT57150.07.81116631
CGGGCAT80450.07.79205751
TAATCGA18500.07.6988384
Produced by FastQC (version 0.11.5)