FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23049305
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGG129850.014.19409650-51
AAAAGGG351700.013.2161580-81
AAGGGGG356950.013.11510982-83
AAAGGGG342500.011.060801580-81
GGGAAAA339550.010.65164622-23
GGGGGAA387150.09.84491720-21
AAAAAAG742000.09.63109378-79
TAAAAAA809950.09.437139570-71
CGGGGAT66050.09.3469651
AAAAAGG496000.09.13116778-79
GGGGAAT157350.08.8588262-63
CCGGTAT23800.08.7796211
CCGGGAT65850.08.6541721
TATAGGG61050.08.01450348-49
GGGGAAA357900.07.92251722-23
CGGGCAT80100.07.82602931
GGGGGGA615700.07.814253360-61
GCGGTAT22950.07.6563031
AGCGTCG213300.07.570875694-95
GCGTCGT185200.07.50141194-95
Produced by FastQC (version 0.11.5)