FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33189778
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAGGGG500550.013.081799580-81
TAGGGGG198300.012.97160650-51
AAAAAGG681650.010.8738178-79
AAAAGGG493950.010.07828280-81
AAGGGGG537300.09.99481382-83
CGCGAAT14500.09.4982851
AGGGGGG923050.09.325109582-83
TAAAAAA1124850.09.29635570-71
TACCGTA25050.08.5301729
CCGGGAT96500.08.3171521
CGCGAGT30950.08.1326191
AAAAAAG1020900.08.04861876-77
CGGGAAT77600.08.0172381
GGGGGAA527700.07.87988120-21
GGGGGGA946400.07.816348620-21
CGGGGAT100800.07.4912071
CGGACAT66550.07.42165041
AAAAAAA6270000.07.38969274-75
TATAGGG83100.07.345573448-49
CGGGCAT111700.07.31291441
Produced by FastQC (version 0.11.5)