FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n02_HFFLm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n02_HFFLm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37442197
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAAAGGG576900.013.65610280-81
TAGGGGG209950.013.65475750-51
AAGGGGG595600.013.135879582-83
TATAGGG106600.011.02917248-49
AAAGGGG572400.010.99168280-81
GGGAAAA565400.010.76499222-23
AAAAAAG1196500.010.40493778-79
TAAAAAA1322350.010.01641770-71
GGGGGAA644200.09.7947420-21
AAAAAGG792400.09.50700878-79
GGGGAAT259800.09.03081262-63
CCGGGAT106700.08.9014141
CGGGAAT86750.08.7040441
CGCGAAT16450.08.6606161
CGGGGAT108900.08.6343711
TAAGGGG167350.08.57242750-51
CGCATAT25000.08.5480281
GGGGAAA602600.08.20094422-23
GGGGGGA1004000.08.16725760-61
TACCGTA27750.08.0427829
Produced by FastQC (version 0.11.5)