FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33051797
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29629178.964465683968712No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT488570.14781949677350373TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC484550.057.566258
TCGGAAG522600.054.4783483
CGGAAGA530650.053.9827884
ATCGGAA535400.053.531742
GATCGGA532900.052.562861
GAGCACA673850.041.677699
AAGAGCA727200.039.6293837
GAAGAGC762300.037.379186
ACTCGAT131700.036.17262336-37
TCTCGTA124700.035.6240842-43
TACTCGA135850.034.8403536-37
GGAAGAG831500.034.7703135
CTCGTAT127900.034.69848344-45
CGTCTGA404400.033.6835716-17
ATGCCGT160150.033.5848248-49
ACACGTC406300.033.5821712-13
ACGTCTG406350.033.53837614-15
CACGTCT406350.033.35159714-15
CACACGT414500.033.03225712-13
TCGATCT128050.032.371738-39
Produced by FastQC (version 0.11.5)