FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24301960
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT805610.33150001069872553TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT493700.2031523383299125TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT451200.18566403697479547TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTTT300960.1238418629608476TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTAT268070.11030797515920526TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA798850.051.1238179
AGAGCAC801000.050.9450878
TCGGAAG817300.049.8262983
ATCGGAA818100.049.7440722
CGGAAGA829250.049.0969164
GATCGGA836450.048.4631231
AAGAGCA900450.045.434137
GAAGAGC973800.042.0282366
AATCGGG319550.041.1024340-41
GAATCGG327600.040.8536840-41
AATCGCG57950.040.28851340-41
AGAATCG397750.040.2818838-39
CACTCCG500200.040.23859430-31
ACTCCGG517050.038.9465232-33
CGGAGAA532750.037.4246636-37
TCCGGAG539600.037.36704634-35
CCGGAGA541050.037.0342634-35
CTCCGGA546800.036.86228632-33
GGAAGAG1132250.036.255695
GTCACTC559900.036.2104228-29
Produced by FastQC (version 0.11.5)