FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25088313
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT654040.2606950893828533TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT407510.16243021202740893TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT372980.14866683144458537TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA743200.046.606669
AGAGCAC744400.046.467548
TCGGAAG763000.045.2745133
ATCGGAA763400.045.133212
CGGAAGA775100.044.5249374
GATCGGA785750.043.7397841
AAGAGCA841800.041.2207227
AATCGCG51400.040.52384640-41
AATCGGG270300.039.99727240-41
GAATCGG282100.039.08194740-41
AGAATCG344750.038.97779538-39
CACTCCG439800.038.8258530-31
GAAGAGC927500.037.4958466
ACTCCGG458850.037.17806632-33
CGGAGAA474600.035.67434336-37
TCCGGAG483350.035.45580334-35
CTCCGGA487600.035.23923532-33
CCGGAGA489200.034.8473334-35
GTCACTC494600.034.81653228-29
ACGTCTG518900.033.34523414-15
Produced by FastQC (version 0.11.5)