FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32004218
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT866050.2706049558842525TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT544490.17013069964715274TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT487480.15231742265972564TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTTT337290.10538923338167488TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC983550.047.5015758
GAGCACA993400.047.183579
TCGGAAG1019900.045.8194773
ATCGGAA1024800.045.536222
CGGAAGA1034550.045.1936654
GATCGGA1049650.044.399711
AAGAGCA1118950.041.8427247
AATCGGG352400.040.276440-41
GAATCGG362850.039.94119340-41
AATCGCG76250.039.90113440-41
CACTCCG583400.039.52320530-31
AGAATCG457050.039.26425638-39
GAAGAGC1217750.038.5938226
ACTCCGG610400.037.7991932-33
CGGAGAA625850.036.52139736-37
TCCGGAG639200.036.20790534-35
CCGGAGA639100.036.00546634-35
CTCCGGA643750.035.9996132-33
GTCACTC661300.035.21907828-29
CGTCTGA697400.033.71101816-17
Produced by FastQC (version 0.11.5)