FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm11.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm11.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24631633
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT650120.26393702764246285TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT402760.16351331639278646TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT364690.14805758107876973TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC744200.046.000878
GAGCACA751950.045.684859
ATCGGAA769300.044.4227142
TCGGAAG770100.044.381743
CGGAAGA779550.043.782894
GATCGGA790900.043.022241
AAGAGCA848900.040.405637
AATCGGG270600.040.0579140-41
AATCGCG51800.039.88962640-41
GAATCGG281500.039.17335540-41
AGAATCG343300.038.9909838-39
CACTCCG435050.038.78608730-31
ACTCCGG451050.037.38981632-33
GAAGAGC926050.037.087296
CGGAGAA466800.035.95543736-37
TCCGGAG473700.035.727434-35
CCGGAGA477350.035.44426334-35
CTCCGGA481350.035.2483732-33
GTCACTC492500.034.5746528-29
CGTCTGA520850.032.9962316-17
Produced by FastQC (version 0.11.5)