FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26373455
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT700580.26563830942893146TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT444400.16850276158356953TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT394550.149601180429337TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTTT268590.10184103675457008TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC790550.047.1308338
GAGCACA801750.046.584999
TCGGAAG813200.045.9196473
ATCGGAA814050.045.755882
CGGAAGA824400.045.198034
GATCGGA834450.044.4857831
AAGAGCA908700.041.117827
AATCGGG292550.040.23540-41
GAATCGG300600.039.69478640-41
AATCGCG56800.039.63992340-41
CACTCCG468950.039.30891830-31
AGAATCG375750.038.62565238-39
GAAGAGC979200.038.304986
ACTCCGG491550.037.5891132-33
CGGAGAA502150.036.5169736-37
TCCGGAG515700.035.94406534-35
CCGGAGA515200.035.69314234-35
CTCCGGA522100.035.54884332-33
GTCACTC538200.034.50211728-29
CGTCTGA563800.033.30451216-17
Produced by FastQC (version 0.11.5)