FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm09.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm09.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24730875
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGTT734040.29681117226948095TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGAT455060.1840048117990164TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT411350.16633054835302025TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGTTT271090.10961601641672607TruSeq Adapter, Index 6 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC800950.047.7174228
GAGCACA808950.047.3219539
TCGGAAG841900.045.4151153
ATCGGAA843000.045.221462
CGGAAGA844650.045.216924
GATCGGA867250.043.8350331
AAGAGCA907100.042.19637
AATCGGG297600.040.5498440-41
GAATCGG306750.039.89000340-41
AGAATCG374100.039.79351838-39
AATCGCG58600.039.47609340-41
GAAGAGC983600.039.0225876
CACTCCG482400.038.951130-31
ACTCCGG497000.037.81286632-33
CGGAGAA509950.036.6676336-37
TCCGGAG516700.036.4916234-35
CCGGAGA518650.036.1895734-35
CTCCGGA524800.035.98631732-33
GTCACTC536250.035.34472728-29
GGAAGAG1155400.033.495565
Produced by FastQC (version 0.11.5)