FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm08.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm08.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25310191
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3846211.5196289905516716TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA914850.049.1431129
AGAGCAC919300.048.9154668
CGGAAGA974700.046.0158424
TCGGAAG978900.045.779413
ATCGGAA983800.045.503932
TCGTATG490850.044.9673644-45
CGTATGC496600.044.69172746-47
TATGCCG498150.044.33889448-49
GATCGGA1009950.044.1330071
CTCGTAT472600.044.044-45
AAGAGCA1032200.043.6801347
TACTCGA510000.042.959136-37
TTACTCG518100.042.81896634-35
TCTCGTA471550.042.65738742-43
ACTCGAT500650.042.1437636-37
ATCTCGT481800.041.173142-43
ATGCCGT541900.040.64525648-49
GAAGAGC1114150.040.490776
GTATGCC553250.039.96096446-47
ATTACTC560650.039.7556534-35
Produced by FastQC (version 0.11.5)