FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm07.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm07.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24446193
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3453361.4126371333156047TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC799050.049.036448
GAGCACA806350.048.7696089
TCGGAAG853250.045.6830523
CGGAAGA859150.045.413934
ATCGGAA858400.045.376332
TCGTATG429700.044.83370644-45
CGTATGC434300.044.60603746-47
TATGCCG435250.044.27501748-49
GATCGGA883500.043.911441
CTCGTAT416700.043.6560244-45
AAGAGCA913050.042.986767
TACTCGA445800.042.89061436-37
TTACTCG455150.042.60963434-35
TCTCGTA412800.042.4682642-43
ACTCGAT433800.042.41823236-37
ATCTCGT425850.040.66486742-43
ATGCCGT481300.039.9845748-49
GTATGCC488650.039.47947346-47
GAAGAGC998100.039.358136
CTCGATC443900.039.3507638-39
Produced by FastQC (version 0.11.5)