FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm06.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm06.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26462388
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3811681.44041422112018TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC907650.047.968278
GAGCACA913100.047.8954059
TCGGAAG969000.044.9031333
CGGAAGA972100.044.7650764
TCGTATG478750.044.71459244-45
ATCGGAA979800.044.3948142
CGTATGC483750.044.3857946-47
TATGCCG483150.044.2502748-49
CTCGTAT459550.043.68846544-45
GATCGGA1009700.042.9050521
AAGAGCA1025200.042.5793047
TACTCGA499000.042.51162736-37
TTACTCG506650.042.37579734-35
TCTCGTA460300.042.2648942-43
ACTCGAT488350.041.92138736-37
ATCTCGT470600.040.80990642-43
ATGCCGT530350.040.22252348-49
GTATGCC541150.039.5768246-47
ATTACTC547500.039.4743334-35
GCCGTCT542650.039.18565850-51
Produced by FastQC (version 0.11.5)