FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm05.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm05.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29721562
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT4087621.3753045684476475TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1005450.048.52169
AGAGCAC1008750.048.301338
TCGGAAG1060950.045.77893
ATCGGAA1062750.045.6481932
CGGAAGA1068300.045.4772724
TCGTATG532000.044.8951244-45
CGTATGC538900.044.6342246-47
TATGCCG540550.044.31041748-49
GATCGGA1093700.044.1262931
CTCGTAT511750.043.61773344-45
AAGAGCA1136200.042.8916327
TACTCGA552250.042.7737236-37
ACTCGAT538200.042.34584836-37
TCTCGTA511700.042.27602842-43
TTACTCG567700.042.25375434-35
ATCTCGT518950.041.0446642-43
ATGCCGT591750.040.348148-49
GTATGCC600550.039.9375646-47
GAAGAGC1240600.039.3678556
CTCGATC545150.039.29282438-39
Produced by FastQC (version 0.11.5)