FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23820144
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3464611.4544874287913625TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC805950.049.0945558
GAGCACA806200.049.0561759
TCGGAAG857500.045.9777643
ATCGGAA859550.045.8519172
CGGAAGA865200.045.629154
TCGTATG431550.045.0649144-45
CGTATGC437500.044.65355346-47
GATCGGA882700.044.478491
TATGCCG437750.044.4339848-49
CTCGTAT418500.043.62678544-45
AAGAGCA910350.043.5268487
TACTCGA450350.042.8691236-37
TCTCGTA412300.042.65240542-43
TTACTCG460550.042.4971534-35
ACTCGAT437600.042.3814436-37
ATCTCGT423600.041.04921742-43
GAAGAGC986600.040.2939226
ATGCCGT484450.040.14082748-49
GTATGCC488550.039.90006346-47
CTCGATC442050.039.5483238-39
Produced by FastQC (version 0.11.5)