FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm03.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm03.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23049305
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT3409921.4794025242843547TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA785700.049.6347859
AGAGCAC785350.049.6140338
TCGGAAG812700.047.8528253
ATCGGAA817300.047.5151022
CGGAAGA821650.047.2912184
GATCGGA840050.046.0393451
TCGTATG425200.044.95558544-45
CGTATGC430050.044.65414446-47
TATGCCG431100.044.40831848-49
AAGAGCA881050.044.3162887
CTCGTAT410800.043.87761744-45
TACTCGA442050.042.8248636-37
TCTCGTA408450.042.7519142-43
TTACTCG451100.042.69723534-35
ACTCGAT432700.042.22987736-37
ATCTCGT417100.041.21613742-43
GAAGAGC949200.041.1665736
ATGCCGT471200.040.63916848-49
GTATGCC479250.039.94107446-47
GCCGTCT479700.039.6384250-51
Produced by FastQC (version 0.11.5)