FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33189778
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT4983311.501459274599547TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC1139800.050.3155028
GAGCACA1148550.050.027449
TCGGAAG1189600.048.0465473
ATCGGAA1191550.047.9649432
CGGAAGA1200150.047.6877254
GATCGGA1223200.046.580241
TCGTATG624700.044.9370944-45
CGTATGC630800.044.7819246-47
AAGAGCA1284350.044.6895457
TATGCCG634450.044.40522448-49
CTCGTAT598500.043.91198344-45
TACTCGA648950.042.95394536-37
TCTCGTA597800.042.5244642-43
TTACTCG665800.042.45865234-35
ACTCGAT636150.042.24648336-37
ATCTCGT603900.041.4617142-43
GAAGAGC1391850.041.283856
ATGCCGT692800.040.54183648-49
GTATGCC699500.040.2852846-47
GCCGTCT704100.039.66325850-51
Produced by FastQC (version 0.11.5)