FastQCFastQC Report
Thu 28 Feb 2019
HW7NHBGX9_n01_HFFLm01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7NHBGX9_n01_HFFLm01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37442197
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT5712381.5256529952021778TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA1309650.049.9987649
AGAGCAC1309300.049.8775758
TCGGAAG1358950.047.9872933
ATCGGAA1361400.047.8220372
CGGAAGA1376250.047.3705254
GATCGGA1395350.046.4683341
TCGTATG712200.044.9044144-45
CGTATGC719500.044.7142746-47
TATGCCG722450.044.33279848-49
AAGAGCA1475600.044.3143167
CTCGTAT688500.043.8456244-45
TACTCGA742400.042.70061536-37
TTACTCG756000.042.62029634-35
TCTCGTA684700.042.59343342-43
ACTCGAT724600.042.13370536-37
ATCTCGT697650.041.23085842-43
GAAGAGC1592950.041.1559756
ATGCCGT790100.040.4888348-49
GTATGCC797750.040.2151846-47
GCCGTCT803800.039.59325850-51
Produced by FastQC (version 0.11.5)