FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16530233
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14884489.00439818361907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTTT137800.024.3918346
GATCTGG133850.020.120686
CGATCTT253150.020.113095
ATCTTTT158950.020.1114857
CTTCACA72750.019.236681
ATCTGGG55150.018.2009837
CGATCTG452650.017.830715
CAGGGGT29050.017.4559524
TTCGGAA20450.017.1042212
CCGATCT1303200.016.468984
TTCCGAT1332100.016.1774832
TCCGATC1352700.016.0587083
ATCTGTG34050.015.7156657
TAGGACT15950.015.3482554
TTCACAT61250.015.1905012
TCTGAGC19100.015.0164718
CTTCCGA1491500.014.7922361
GTCTTAC17050.014.77444651
ATCTGCG42650.014.7608697
GTCCTAG12650.014.65846351
Produced by FastQC (version 0.11.5)