FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3119698
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG120350.3857745204824313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG173850.030.1609125
GATCTTT125900.028.9681976
TTCCGAT537100.027.6990092
ATCTTTT128500.027.2912357
CCGATCT544300.027.0186394
TCCGATC553550.026.828583
TCTTTTT131300.026.682598
CTTTTTT133700.026.0989079
CGATCTT187650.025.4805895
GATCTGG76250.024.649836
ACTTTAG7550.024.584371
ATCTGTG12800.024.0619777
TTTCGGA12100.024.0227581
ATCTGGG33550.023.8891967
GATCTAG34200.023.7433176
GATCTGA59800.023.2949286
CTTCCGA645600.023.266051
ATCTGAG22450.023.0729947
CGATCTA100900.021.8552975
AGGTGTA27550.021.47199670
Produced by FastQC (version 0.11.5)