FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s95.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s95.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33.1578947368421053No Hit
CCAGACAGATCAGTTGCGTTCTATGCCAGTGCAATCGACGCTCTGGAGAA11.0526315789473684No Hit
GCCGAGATGGGATGGGCAGAGGTCTCCCGGGGGAGGTCGGAGGGGGACAG11.0526315789473684No Hit
CTGAAGTTAGACGACTGATCATATGCAAACTCGACGGATCCTAGTACACT11.0526315789473684No Hit
GAGCACACGTCTCCCACCGTAGATCGGAAGAGCACACGTCTCCCACCGGA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGGAGGGCGGCAAAGCGGCGGACTGGGGCCAGGAGCCAGTTTAAAAGAC11.0526315789473684No Hit
GTGCTCTTCCGATCTATATAGGGACTGTCTCTTATACACATCTGACGCTG11.0526315789473684No Hit
TCCACTGACAGATCGAAAGAGAACACGTCACCCAGCAGAGCGAGGAATGA11.0526315789473684No Hit
GTCGTGTGTAGCTCAACCTTACTCGCGTGTCCGGGTAGCAGGGTTACACT11.0526315789473684No Hit
GCCACATTCAGTCCAGCACAGCACGTATCACGCGAGCTGACGGGGTGGTA11.0526315789473684No Hit
CTTTTGTGAGTCTCCTGTCTCATTCTTACAGCTCCATGAAAGCAGGAACA11.0526315789473684No Hit
CTGCAGATCTCGCTATGGAGTACGGTTCTAAACCGATAACTCTAGTGTTC11.0526315789473684No Hit
TCCCTGACCAGATAGTAAGACAAAACATCACCCTGACCAGAACTGAAGAG11.0526315789473684No Hit
CTGCACATCCGAAAAAAAAAAAAAAAAAAAAAAACAACGAAGCGAACGTC11.0526315789473684No Hit
CTTCCGATCTAATCAGGGAGACGTGTGCTCTTCCGATCTACACTGGGAGA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CACCAATACCAAGCTGTAGTAAAGGTCCCGGGGTCTTTCCGTCCTTCTGC11.0526315789473684No Hit
GCATTTATCTTTGATTTAGATGGCACACTGATTGATAGTGCGCCTGATAT11.0526315789473684No Hit
CAAACGATCTCAGAGTGGAAACGTGTGATCATCCGAAAAAAAAAAAAAAA11.0526315789473684No Hit
ACGCCGGTGGCCGGGCGGGGTGGCGGCCGTCGTTGGGGCGCTGGGGCGCG11.0526315789473684No Hit
TCTCAGACGTGTGCTCTTCCGATCTCAGACGTGTGCTCTTCCGATCTCAG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CAACGGCCAAATACTAACAGCAGACATCTCAAGAGCACAATAATACCAGC11.0526315789473684No Hit
GCTACTATACAGTCAAAAATTCGAGACATCGAAGAAACGCCAACTTACAT11.0526315789473684No Hit
ATCAAGAGCAGATAGAAACATCACAGCACTCCAACTTGATATAATAAAAG11.0526315789473684No Hit
GAGTAACGAAGACAGCTTCACCGTCAGCCCGTTGCGGTCGATGCGCAGCA11.0526315789473684No Hit
GAGCACAGGTCTGCGGATGTTAGGTTGGAAGCGCCCACGTCTGCGCATGT11.0526315789473684No Hit
GCAGACGCGGTTATACGAACTGGGCGAACGTTGAATGAATAAATAGGTTT11.0526315789473684No Hit
ACATAGCTCATTAAAGGGGTGAAATCGAGACGCAAAGGATCAAACCGCGA11.0526315789473684No Hit
TCCCTGCTGAGATCGGAAGAGCACACGTCTCCCTGCTGAGATCGGAAGAG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTCCGATAGACGTGTGCTCTTCCGATAGACGTGTGCTCTTCCTGTCTCT11.0526315789473684No Hit
AACAAATGGACATAGCAAGACAACACCTCTACCAATGGAAATAACAAGAC11.0526315789473684No Hit
TTTTTTTTTTCGGAAGAGCACCCCGGCCAGATCGGAAGCTGTCTCTTATA11.0526315789473684No Hit
ATGTTAGGTTCAGTCCGCTAACGAACGCAATCCATGGAAGGAATCAATTC11.0526315789473684No Hit
GGCTAAAGTTTTCGCAGCTTCGGCGGTTCTCAGGCGATGCGACGATCGCT11.0526315789473684No Hit
CTTCTCGTCCGAGGTTTTTTTTTTTTTTTTTTTTTTTTTTTAGGGGAAGG11.0526315789473684No Hit
TAACGATACTTATACAATATCTGTAACACTCACTATTAAGGATTACAAGT11.0526315789473684No Hit
TCCGAGACGTGGGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC11.0526315789473684No Hit
ACAAAAAAAGATCAGAAAAACAAACTTAACACTATACACATAGTAACAGC11.0526315789473684No Hit
GCCTCGGGCAGGGCGCTATGGGGCGAGGACGCTGTGGCGTGTGATACCCG11.0526315789473684No Hit
CTTCGGAAGATCACACGTCTCAGAATGAAGAACGGAAGCTCACACGTCTA11.0526315789473684No Hit
CACTTACCCTCACCGCCTGATTACCAACCAGGCTGAGGGAACCTTTGGGC11.0526315789473684No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGGGGGGGCCGGGGGGT11.0526315789473684No Hit
AAAATACACATTACAATAACACGACCTCGCATAAAAGTACTGTAAAGTTA11.0526315789473684No Hit
ACGTGGGTCTGCCAGAGAGAACTCTGAACTTGGCCAAGACGAGAGAGCGA11.0526315789473684No Hit
GAGCACACGTCGCCCGACCGAGATCGGAAGAGCACCCGTCGCCGGGGCTA11.0526315789473684No Hit
TCAGAGCACTGGGCTGAATTCACATTGCGTTAACATCCGTTGGGACCATC11.0526315789473684No Hit
GAAGCTCTCTTTTGATAGAGCCGTTTTGAAACACTGTTTTGGTAGAATCT11.0526315789473684No Hit
GTGCGACATCGACCATTTCAAGTCGATCAACGACCGCTACGGACATGAGA11.0526315789473684No Hit
ACGCAGGAGAACTGAGCAATTCAGATAAGACTTGGGGTTATCATCAGAAA11.0526315789473684No Hit
GAGTGTGCTCTGAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGA11.0526315789473684No Hit
ACACCATCGTCGTCGGCCCCCCCGTGCTGTATGGCGCGCTGGCCGGCGAG11.0526315789473684No Hit
GACACAGACATGGAGTCCATAACAGGACTGTTTCCTGGTGCTCAGGGGCT11.0526315789473684No Hit
ATGTAAAGGGCGACGATGTTCATCACCAGCGTCAGCGCGAACAGCGTGAT11.0526315789473684No Hit
CGTCTCCCAAATCAGATCGGAAGAGCACACGTCTCCCATATCAGATCGGA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCCCTCTTTAGATCGGAAGAGCACACGTCTCCCTCG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGTGGAAGGTTGTAGGCATGTATCTTAGGCAAATCCGGGGTACTCTATGC11.0526315789473684No Hit
CCCCTTAAGAGATCGGAAGAGCACACGTCTCGGTTAAGATCGGAAGAGCA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGCGTCTGCCGCGGTTTATGGGGTGGGCTGATGGGGGCCGTGGGTGATCG11.0526315789473684No Hit
GGATTACCCCGAGCGGGACATCCAAGGGGCGCTTCTTGCGGGGGTAAAGG11.0526315789473684No Hit
ATATCGGAGATCGGCTGCACATCCAGCAAGGCGGCCACGCGCGGCAGCAC11.0526315789473684No Hit
GGGGAGACGTGTGCTCTTCCGATCTCGCTGGGGAGACGAGTGCTCTTCCG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGCGTCGAGGAGGGGGGGGTCGGGCCTGTGGCGGGTGCGGACGCTGCGC11.0526315789473684No Hit
CGTCTCCCAGAATAGATCGGAAGAGCACACGTCTCCCAGAAAAGATCGGA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCTCAGATCGGAAGAGCACACGTCTCAGATCGGAAGAGCACACGTCTCCC11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
CACGTGAGGAACTTGCCCATAAGAGGGGGATAACACCCGGAAACGGGTGC11.0526315789473684No Hit
TCTCCAGCGTGGGCGGGCGCCCACGGACGATGCGTTCCTGGCTCGCGATG11.0526315789473684No Hit
CTCACACGTGAGACGTGTGGTGGTCCGATCTCACAGGGGAGAAGTGAGCT11.0526315789473684No Hit
CAGCGATTCCAAGCAAGAATCGTACAGTACTCACTGGCTCAGTTGCCACT11.0526315789473684No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11.0526315789473684No Hit
GTATCGAGCGCGATCTCGCCGCTGCGCGCGGAGACGTAGAGATCGGTGCC11.0526315789473684No Hit
CCCAAAGGTTCCCTCAGCCTGTTTGGTAATCAGGTGTTGAGTGTAAGTGC11.0526315789473684No Hit
GGTGGAGGTCACCACCCCCGAGGAGTACATGGGCGACGTGATCGGCGACC11.0526315789473684No Hit
CTCTTCCGATCTACTCTGGGAGCCGGGTGCTCGTGGGCGCTACTCTGGGA11.0526315789473684No Hit
GGGCGGGGGGGGGTGGGGAGACGTGTGCGCTGCGGAGCTGACGCGGCGGG11.0526315789473684No Hit
CTCGTAGACGTGTGCTCTTCCGATCTGAATAGGGAGACGTGTGCTCTTCC11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTGTAGAATATGTATTTAACATTAGGATTTCAGAAGCATGAGAAAAACAC11.0526315789473684No Hit
ATTCGGAAGAGCACACGTCTCCCACTGCCTGTCTCTTATACACATATGAC11.0526315789473684No Hit
GTCCTCTTCCGATCTTCGCAGGGAGACGTGTGCTCTACCGATCTGCGTAG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGGGGCGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11.0526315789473684No Hit
CGGTACTGGTTCACTATCGGTCACTAGGGAGTATTTAGGCTTGGGAGATG11.0526315789473684No Hit
GCCATCAACGCCGTCGATGCCAACCAGATCAAGCGCACCGGTTCGCTCAA11.0526315789473684No Hit
GAGCACACCAATCCCATGACAGATCGGAACAGGTCTCGAATGCCATAACG11.0526315789473684No Hit
TCCGATCTGTCAAGGGAGACGTGTGCTCTTCCGATCTGTCAAGGGAGACG11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTTGGGGGGGGGTTGTTTTTTTTTTTTTTTTTTTTTTTGGTGGGGGGGGC11.0526315789473684No Hit
CCACTGGGCTGAGTGGGAAAGGATGTGGGTTGCCCAGACAACCAGGATGT11.0526315789473684No Hit
CTTCCGATCTGTGTAGGGATTGTGTCGTTCATCCTTATATTCTCAGTGCT11.0526315789473684No Hit
CTTCCGATCTGGACTGGGATAGCGGCATTGCCGTAATTCTCGGGCTTCGC11.0526315789473684No Hit
CTTCCGATCTCAACAGGGAGACGTGTGCTATTCCGAACTCAACAGGGAGA11.0526315789473684No Hit
GGGGGGAGTCGGCTGCACGACCGCGATCGCAAGATCGGGAGAGTGGTGGA11.0526315789473684No Hit
TATCGGAAGAGCACACGTCTCCCGGGGAAGATCGGAAGAGCACACGTCTC11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTAGGCCCCAAAGACCACCACGTAGTAGAGGCTGAAGCGCCACACCCGT11.0526315789473684No Hit
AACGGGTTCTCCCCGGAAAGGAAAACCCGTTCCTTGGTGAGATCGAATTT11.0526315789473684No Hit
GGGGGGGCGGGGGGGGGGGGGGGCGGGGGCGGGCGCCCGGTGCCGGGGGG11.0526315789473684No Hit
GTCTCCCACTTTAGATCGGAAGAGCACACGTCTCCCACTATAGATCGGAA11.0526315789473684Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTTGGG50.070.029
GGAGAGA50.070.065
CGGGGGG50.070.043
GGCCGGG50.070.040
AGAGGGG50.070.069
TTTTTTG50.070.027
TTTTTGG50.070.028
GTTTTTT50.070.01
GGGAGAG50.070.064
TTGGGGG50.070.031
GGGGGGC50.070.036
GGGGGGA50.070.061
GCCGGGG50.070.041
GGGGGCC50.070.037
GGGGGAG50.070.062
AGAGAGG50.070.067
TTTGGGG50.070.030
GAGAGGG50.070.068
GAGAGAG50.070.066
CCGGGGG50.070.042
Produced by FastQC (version 0.11.5)