FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42.5974025974025974No Hit
GTTGTCGTCAGCTCGTGTCGTGAGATGTTGGGTTAAGTCCCGCAACGAGC21.2987012987012987No Hit
CTCTTCCGATCTTGCACGGGAGACGTGTGCTCTTCCGATCTCTAATGGGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
AGCCCCGGCGGCTCGGGAGGGGGGATTCGGGCTCGCTTTCGCTACGGCTA10.6493506493506493No Hit
CTTCCGATCTGCACCGAGACGTGTGCTCTTCCGATCAGACGTGTGCTCTT10.6493506493506493TruSeq Adapter, Index 2 (95% over 21bp)
GCGTGCGCGTTGGCGCCGGTGCGCGGCTTTTCGTTGGTGGGGGCGCCGAG10.6493506493506493No Hit
CTTCCGATTCTCGCCTGCCCGTCACCGTGTTGTCCGGCTTCCTGGGCGCC10.6493506493506493No Hit
CCGGGGGGCCGTGGGCGCTTCCGCGCGCCCCGGGGAGCCGGGTGCTCGGC10.6493506493506493No Hit
GGTCCTCGTTGGTGAGGGCGCGGGGGGCGCGGGCGGGGGGCGGGGGGGTT10.6493506493506493No Hit
CTGTCTTGGGAGACGTGTGCTCTTCCGATCTGCCTTGGGAGACGTGTGCT10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCCGATCGGGATTGGGGGGCGGCCTCGGCGTTCTGCAGCTCGACCAAC10.6493506493506493No Hit
GGGGGGGGGGGGGGGGGGGCGGGGGCGGCGGGGGGGGGGGGGGGGGGGGG10.6493506493506493No Hit
GTACTGCTACGTCTGAATATGGACAAAAAGCGGAATAAGGATTTGACCCG10.6493506493506493No Hit
GGCGAGGGTGCGTTCCCGGTTGAGGGACAGGTGGGGTGGGCGGGGTGGGG10.6493506493506493No Hit
GAGCCCCCCAAGAGAAAAACTAACATCACCCCACTCCCAACCACGAACGA10.6493506493506493No Hit
GGGCGGGAGGGAGGCGGGACGGTGGTAGGGGGGGAGGTTGTGGCGCTGCT10.6493506493506493No Hit
TAAGTTAGCGGATCGGTGAGCACTTACTCAATGCCCGGTGATGGAAGGAA10.6493506493506493No Hit
GGATGCGATGAGCCGACATCGAGGTGCCAAACCTCCCCGTCGATGTGAAC10.6493506493506493No Hit
ACTCAATGTGGTTAGGCGAGTGCGTAATTAAAACTACCTAGGTGCTCTTC10.6493506493506493No Hit
ATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGAAGAACCCCCCC10.6493506493506493No Hit
GTTTGCTCCCCACGCTTTCGCACATCAGCGTCAGTTACAGACCTGTCTCT10.6493506493506493No Hit
GATCCTCTTACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACAAAAAA10.6493506493506493No Hit
GCAACAAGGATCGTCGGATGAGGATCGCTGCTGGCGCTGGTCTCTCGGAG10.6493506493506493No Hit
CACCTATCCTAATCAGTACCCACATCTATTAAAAAGCTACTAGGTTGTAC10.6493506493506493No Hit
TATTAATACCATAATTAACAACAAACACGACATCATACCTGCCAACATGC10.6493506493506493No Hit
AAGCAAGGATATAGGCGCGCGCGCCATTTTCGGCAGATGGGCTGTGCGCT10.6493506493506493No Hit
GTGCAAACCGGAGGAGGACCGATCAACCCAACCCAACGTCCAACTACGAG10.6493506493506493No Hit
TCCCGGGTGAGATCGGAAGAGCACCTGTCTCTTATACACATCTGACGCTG10.6493506493506493No Hit
GAATACGCACGTTGAAACGGATCTTGCCTCTTTCCATATCTGTTGATAAT10.6493506493506493No Hit
AGCACAAGACTAACTGTGAAGATCGGATAATAACCAGTATAACAGATTAA10.6493506493506493No Hit
CCCTTGCTAGATCGGAAGAGCACACGTCTCCCACGGTCTGTCTCTTATAC10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAGTTAGCTCATGAGTGCCGCAGGATTCTGCGAGGTTAGGAGAACAAGAG10.6493506493506493No Hit
TCACAGACCTGTTATTGCCGCGCACTTCCATCGGCTTGAGCCGATAGTCC10.6493506493506493No Hit
CGCTTTATCTACGACTCCCCCACCACGGGTTAACCTCGCCACCCATCACT10.6493506493506493No Hit
GTCAAATGCCCTTCGATCAGCTAATGCAGCAATCGATACCTGATTCCATG10.6493506493506493No Hit
GGTCAGAGGAATGTGGCATCTTCGGATGTGTTATAGCCTCTGACTGCATG10.6493506493506493No Hit
GTGGTGGGAGACGTGTGCTCTTCCGATCTGTGGTGGGACTGTCTCTTATA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGTGAGAATCCTGTTCGCCGTAAGGACAAGGGTTCCTGGGGAAGGGTCGT10.6493506493506493No Hit
TCTGTGTCCCCACCAACTCTCATGTGGAATTGTAACCCCCAGTGTTGGAG10.6493506493506493No Hit
GTAGTAGGAGGTGCCAGGTGTCAGGGGAGTGCCATCGTCCAGGGAAGTTA10.6493506493506493No Hit
CATCTGGGGCTGCCGACGACTGCTTACGTGGAGAGCTCTGTGGTGGCCGT10.6493506493506493No Hit
CTTTAATCGTTGAAAAAACGAACCCCCAGACTAGATCGGAAGCTGTCTCT10.6493506493506493No Hit
GACGTGTGCTCTTCCGATCTCACTTGGGAGACGTGTGCTCTTCCGATCTC10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACAACATCAGAACGGCACACCACACCACTACAAACTCAGATCGGAACAC10.6493506493506493No Hit
CTCTCGTACTAAGGATAGCTCTCTTCAAATCTCCAACGCCCGCGACGGAT10.6493506493506493No Hit
ATCAAGGAGCGTCAACAATGAATGCCACGTCGCCGCTGTCTCCGGTCATC10.6493506493506493No Hit
CTCCCTGTAGAGATCGGAAGAGCACACGTCTCCCTGTAGAGATCGGAAGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CTTTTACCGATGTGCCGGTGCCGACGAGTCCTTAGGTGTAGATGGCGGTG10.6493506493506493No Hit
GTTACAGATAGGAAGAGCAAACGTATCCCATTCGAGATCTGAATAGCACA10.6493506493506493No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGGGG10.6493506493506493No Hit
CTTCCGATCTAAACAGGGAGACGTGTGCTCTTCCGATCTTCCATGGAGAC10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGCCCAGCCC10.6493506493506493No Hit
GGGCGGGCGGGGGCGGGGGGGGGGGGGGGGGGGGGGCGGCGCGGGGGGGG10.6493506493506493No Hit
CCCCAGAAAGATCTCTCCAGAATAGAAAAGCCCTCGACGAGTGCCTTGAC10.6493506493506493No Hit
GGCAAGGATCGAATCTCGCGCCACTGGTACGACCTATCCAGGCTGCTCAA10.6493506493506493No Hit
GTGCTCTTCCGGTCTAGACGGGTGGTCTTCCGATCTAGACGCTGTCTCTT10.6493506493506493No Hit
CTTCCGATCTGACCAGAGACGTGTGCTCTTCCGATCTGTATAGGGACTGT10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGTGAGTGTGTGAACCGATCATGTAGTAGGCAAGCTGCGGAGGGACGCA10.6493506493506493No Hit
GTGCTCTTCCGATCTGTCAAGGGAGACGTGTGCTCTTCCGATCTGTCAAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CACAATACATATCCCAATATAGATCGTAACAGAAAAAATCTCCCAATACA10.6493506493506493No Hit
GTTCCGAACTGAACAGGGTGAGGTGTGCTGTGCCCAGCTCAGGGCCGGGA10.6493506493506493No Hit
TCACACGGGAGACGTGTGCTCTTCCGATCTCACACGGGAGACGTGTGCTC10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ACAATGCTTCAATCCAATGATCGTCGAGCTTGCGGGAAAGGCAATGAAAG10.6493506493506493No Hit
ATCCAATACAGCGAGGGTAGTATCCCACCAACGCCTCCACGTAAGCTAGC10.6493506493506493No Hit
AATTAGCAGGGGACAAGAAGGACAGCGGCCAGGTCAAGGCGACGGTCAGG10.6493506493506493No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTATTGGGCGTAAAGCGCGCGTA10.6493506493506493No Hit
ATCTTATAGAGCGCATGCCAAGCGGCCCTGGGAACGCTCAGCGTTGCCAG10.6493506493506493No Hit
GGAAGATCCAGTGCCAGGAGAAATAGGTGGTGATGAAGCCGCCGAGCGGC10.6493506493506493No Hit
TACCTCTACAGAACTATAAGACGATGCCGAAGAAAAAAAACTATTAAATC10.6493506493506493No Hit
CTTTTCTTCTTGACGGCCTATCACCCTGAAATCGGTTAGTCCGGAGCTAG10.6493506493506493No Hit
AGATACGGCGGTCAATTTCGCCGCCAACATGTTTAGACGGGCCGACGCTT10.6493506493506493No Hit
TCCCATAACAGATCGGAAGAGCACACGTCTCCCATAACAGATCGGAAGAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GATGGGGCGCGGGCGGGGGTCCCGTGGGAGGGGGGGGGCGCCCGCGGGAC10.6493506493506493No Hit
CTTGCGATCTGTTTTTTTTTTTTTTTTGGTTGTGTTTTGGGGGGCGCGGC10.6493506493506493No Hit
CTAATACGGGAGACGTGTGCTCTTCCGATCTGCAAAGGGAGACGTGTGCT10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GAAAAAAACAGATTACTTCAGAAAAATTATATATAACTAGAGAAGCTGAC10.6493506493506493No Hit
GATAATATCAATACAAACATACACTCTATGAGACAGAGCCCAAATCGTAA10.6493506493506493No Hit
CCCTTGGAAGATCGGAAGAGCACACGTCTCCCATAGTAGATCGGAAGAGC10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCATTGTAGATCGTAAGAGCACGCGTCTCCCATTGTAGATGGGATGAGCA10.6493506493506493No Hit
CGTCAGCACCGTCAATCTCGACCGTTTCGCCCGGGTAGTGAAACCGGGCG10.6493506493506493No Hit
GCTATGTTTTAATTAGACAGTCTGATTTTGTCCGTACCAGTTCTAAGTTG10.6493506493506493No Hit
ACAGCAGCGTGCTCTTGCCGCAGCCGGAGGGGCCGACCACCGCCACCACC10.6493506493506493No Hit
ACACAGATCGGAAGAGCACACGTCTCCCTACACAGATCGGAAGATCACAC10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATTGCAGACTGCAGCGTTCTGAGAAACATCTTTGTGATGTTTGTATTCAG10.6493506493506493No Hit
CATCTGACGCTGCCGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT10.6493506493506493Illumina RNA PCR Primer (100% over 25bp)
GTTGTCGTCAGCTCGTGACGTGAGATGTTGGGTTAAGTCCCGCAACGAGC10.6493506493506493No Hit
GACGTGTGCTCTTCCGATCTTCAAAGGGAGACGTGTGCTCTTGAGATCTT10.6493506493506493No Hit
GTTCGCGGCGCTCCGCTCCAGGGATCTCGGCAAGTGGATCGCCGATAACA10.6493506493506493No Hit
GGCCGTTGGGGCTGGGCCGCGCGGGGTCTGACCGGGGGGGTGCGGCGCGT10.6493506493506493No Hit
CTTCCGATCTATGCCGGGAGACGTGTGCTCTTCCGATCTATGCCGGGAGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CACCCCTTGCGAGATCGGAAGAGCACACGTCTCCCAAGCCAGATCGGAAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CACTGTAATCAACCCAATCGCACACAACTCCCTTACCAGCTGAAACTAAA10.6493506493506493No Hit
AGGGAGACGTGTGCTCTTCCGATCTGTCAAGGGAGACGTGTGCTCTTCCG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC10.6493506493506493No Hit
GCTGAATACTGACGCCCCCGACTATCCCTATTAATCATTACGGCGGTCCT10.6493506493506493No Hit
GTGCAGGTAGCCCGGCATGTAGGTCTTGAATGGCTTGGAAGCAGGCGCAT10.6493506493506493No Hit
GCCCAGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCATTA10.6493506493506493No Hit
TCCGATCTCCGCTGGGAGACGTGTGCTCTTCCGATCTGAAATGGGAGACG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
ACATAGTCATGCGAGGCGAACCGGGTGAACTGAAACATCTCAGTAGCTCG10.6493506493506493No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC10.6493506493506493No Hit
GGGTGGTGGGGGCCGGCCCGGGGGTGGGGGGGTGCTCTTGGCGGTCGGGG10.6493506493506493No Hit
GACATGGACAAGAGAGCTCTTCCGATATGCCATCAAGAAGTATCGTCAAA10.6493506493506493No Hit
GGTGAGGTGATATGAACCGTTATAAGCGGCGATTTCCGAATGGGGAAACC10.6493506493506493No Hit
GTAGACGTGTGCTCTTACTATCTAGCACGGAGACGTGTGGTATTACCATC10.6493506493506493No Hit
AATCATAGCTAAGTTAGGCGAACAGGGAGAACTGAAACAACTAAGTAACA10.6493506493506493No Hit
ATCAAATGTGAGACATGTGCTCTAAAGATATAAAAAGCGTTACCAGATGT10.6493506493506493No Hit
GGCTCGGGGTGGAGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGCGCGGCG10.6493506493506493No Hit
TTTTTTTTTTTTTTTTTATTTTTTTTTTATGAAGAACCCAACAGAAAAAT10.6493506493506493No Hit
CGTCTCCCTTGACAGATCGGAAGAGCACACGTCTCCCCACGTAGATCGGA10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTAGTGAGATCGGAAGAGCACACGTCTCCCTACAAAGATCGGAAGAG10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACAATCAAGTTGAGCGCCAGAGTCCCCAGGCCGAGGAACTCATCGATGCC10.6493506493506493No Hit
GAACTGCGTCTGATGGGAACTAGGAGCGCCGAATTCATAAAAAGCCAAAG10.6493506493506493No Hit
CATCCGATCTTTTTTTTTTTTTTATTTTTTTTTTAAAAAAAAAAAAAAAA10.6493506493506493No Hit
CGCCAAGGCCGCAGCCCCGGCTGCTGCTGCTCCCGCTCCGGCTGCAGCTG10.6493506493506493No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGAAACCAC10.6493506493506493No Hit
ATCCCACCGACAGCAACAAGGCGCATCCGCTGCCGCTGTCGCCCAACTAC10.6493506493506493No Hit
GCAGAGGGAGACGTGTGCTCGAGCGATCTGCAGAGGGCCAGGGGGGGGGA10.6493506493506493No Hit
AAATTCAGACACACCTGAACAACTGGACCCAAATTATAAATATACCAGCA10.6493506493506493No Hit
CTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTAAGAGGCGGG10.6493506493506493No Hit
TCTCGGTCCAGGCCGTGGTGCTGAACCTGCTCCAGGACCTCAAGGCCCGG10.6493506493506493No Hit
GACGTGTGCACTTCCGATCTGCAAAGGGAGACGTGATCAAAACCCAAATG10.6493506493506493No Hit
GGCGGGGGGGGGCCGGGGCGACGGCGGGGGGGGGGGGGCGGGGGGGGCGG10.6493506493506493No Hit
TGGGAGACGTGTGCGGTTCGGGTGGGGATGGGGAGACGTGTGGGGGTAGG10.6493506493506493No Hit
CGTCTGGGAGACGTGTGCTCTTCCGATCTCGTCTGGGAGACGTGTGCTCT10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GTCCAATATTGAGAGGGTGAAGCTGGCAGGGCTTTGTGATTGGTTGTAGG10.6493506493506493No Hit
CTAGATGATGATCCCCTGCACGGACACTCCCAAACCACGGTACAATCCAA10.6493506493506493No Hit
GGGGTGGGCGACGCTCGCGGGCGCCTCGCTGCTGATGCTCGACGAGCCGG10.6493506493506493No Hit
TCACCGAACACCACTCGTCACAGAAGATCACACGTCGCGCAATCGCACAC10.6493506493506493No Hit
ACAAAAAGTAAAAAAAAAAAAATAAAAAAATAAAAAATACAGATGTGCAC10.6493506493506493No Hit
ATTCTAATCAGATCTCGGATACGAGTGGTGTTCTGTTCCGATATCGACAT10.6493506493506493No Hit
GTGGAATTCCATATCGGTGAAATGCGCAGAGATATGGAGGAACACCAGTG10.6493506493506493No Hit
CCACTGGAGATTTTAGAAGAGAAAAATCTAATAACATATGTGGTTGCTTA10.6493506493506493No Hit
GGTTTTGGTTGATCGGGCCGATCAAGGCGGTGCTCTTCAACGCTTGGGTG10.6493506493506493No Hit
GATCGGAAGAGCACACGTCTCCCGTGGGAGATCGGAAGAGCACACGTCTC10.6493506493506493Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CCCGGGCGAGGTCGGGAGCGCGGCGGGCTCGCTGGCGCGGTAGGGAGAGC10.6493506493506493No Hit
CACTTAGCCCGCTTGGGTTACGGCGTGGACTACCAGGGTATCTAATCGGG10.6493506493506493No Hit
CCTAACGCTGTACCAACGACAACTGTATTTTATATAAAGTAGCTAGCGCA10.6493506493506493No Hit
CCCATGAACAGAATATTCAGGCATTTGAAAGCCTGCTTTTAAGCCTGTCT10.6493506493506493No Hit
CTGAAACGAAATCGGACTCACAAAAACACAAACCAGCTACTCTAACAACC10.6493506493506493No Hit
CTACACATCTAACCTGGGAGACGAATTCTCAAAACAACTAATCAATGAGA10.6493506493506493No Hit
GTTCTCGTCGAGATTATCCTTGCCTTGCTCGGTGATGCTGTAGAGCTTGC10.6493506493506493No Hit
CTCTAATGGGAGACGTGTGGGCTTGCGGTCTCGATTGGGAGACGTGTGCT10.6493506493506493No Hit
GGGGTCGGGGGTCCCGGGGGGGGGCGGGGGGGCGGGGGGGGCCGGGGGGG10.6493506493506493No Hit
CATCTGAAGAGCACAACACAAAAAAAAAAAAAAAAAAAAAAAAAAATCAA10.6493506493506493No Hit
ATGCCCACGAACATCAGGATGTTGGCTATCATCGAGAAGGGCGTGAGGAA10.6493506493506493No Hit
AAATAGGACTAACATGGGTAAAAGCCGTAAACGGAAATCAGGGAACGAAA10.6493506493506493No Hit
GCATTCTAGTATCCAACTCGACTACATGAAGCTGGAATCGCTAGTAATCG10.6493506493506493No Hit
AACAATAACGGTCCTAAGGTAGCGATATTCCTTGTCGGGTAAGTTCCGAC10.6493506493506493No Hit
ATTGTGAGCGGAGAGTGGTGGCATGGACTGGGAATGCGCAGTAAGTGTGA10.6493506493506493No Hit
GTGGTACTACTCGGGTAAACACTACTGCTTCACGATGGGCGCTCACGTGC10.6493506493506493No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers