FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2531371
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79540.3142170784132393No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC64610.25523718174854654No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC50320.19878555928783256No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA49980.19744241361696882Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC48690.19234636092457408No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC45690.18049507559342348Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC45390.17930994706030842No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA5850.025.7447451
TAGGGGT4750.025.0580224
ATCTTTT45900.024.3245647
CTTTTTT44550.024.275569
TCTTTTT45650.024.073958
ACGTGTA22200.021.91609670
GATCTTT57200.021.355696
ACGATAG5650.019.82453269
TTCGGAA7550.019.4797742
GACTCCT112250.019.33333470
AGGGGTG18750.019.2300425
CGTGTAG14600.018.9398169
GTATTAG3250.018.320681
CGACTCC134150.016.88165369
TAGGGTG4150.016.8704325
TACGTGT30450.016.66794669
CGTGTGG16700.016.55815770
GTATATG2351.532535E-716.39461
CAGGGGT7950.016.292814
TCCTACA3701.8189894E-1216.0889912
Produced by FastQC (version 0.11.5)