FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33.8461538461538463No Hit
CGAATTAGCCGTGACCTTCTCCGAAAAAACAACTAGCCCTTCAGAAAAAA11.282051282051282No Hit
CTTCCGATCTAATGGGGGGGCCCTGGTGCATAGGCTGTGTTCCTGTCTCT11.282051282051282No Hit
AGCGAACCCAGGATGCTGGCGGCCTCGATCGGAAGCATGATGATCTTCTG11.282051282051282No Hit
TGTCAGGGAGACGTCTGCTCTTCCGATCTTGTCACGGAGACCTGTCCTCT11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CATACATAAAGGTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTA11.282051282051282No Hit
GTACACACGAATTAAAAGTGAAACCGCAAAAGGCTCATTATATCAGTTAT11.282051282051282No Hit
GCTAGAAAGAAAGGAACCGTACCGCAAACCGACACAGGTCGTCGAGATGA11.282051282051282No Hit
TATCAGGCGTATGCTCTTCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
CGGTGAGTGGGCGCGAGCGTGGCCGGGCGGTCGCGGGGGGGGGTGCGCGG11.282051282051282No Hit
TGCTGGAATAGGGTGAGGCCAGAGGAGGCTCTGGGGGAGGCTCGCAGCGG11.282051282051282No Hit
ATCTAAAACCGGGTAAAGTAAAAAATATAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
AATAACTCCAGACCGCCACTATTCTAGCCGATGATATACTGATAGATTTT11.282051282051282No Hit
GTGGTGGGAGACGTGTGCTCTTCCGATCTGTGGTGGGAGACGTGTGCTCT11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CATCTGACGCTGACGACGACTCCTTACGTGTAGATCTCGGTGGTCGCCGT11.282051282051282Illumina RNA PCR Primer (100% over 25bp)
GCCGGGGGGGGGTGGGTGGCGCCCGCGGGGGGCGGCGGCGGGCGGGGGGG11.282051282051282No Hit
GCCACAGGCAGAGCATCGGCGCTGCGTCCGACGAAGGGCCGCTACCGCCG11.282051282051282No Hit
TGTTAATTGAACATTATCAACCGTGTTATTACCGGTGATTGTACCTGTAC11.282051282051282No Hit
GCCCGCGGCGGGGCAGGGCCTTGGGCATGGGCAGCACTGCGGGGTGACGG11.282051282051282No Hit
CTAAAAATATAAAAAAGTAATATAACATGAAAACAATCATAATCTTAAAA11.282051282051282No Hit
TCGCCAGCGTCTCGATCTTGCTGGTGAAGATTTTCCGCCGCGCGTCCGGC11.282051282051282No Hit
GTTATGCCTCGGGGAGTTGTCAATCAAGCGTTGATCCGAGGATGTCCGAA11.282051282051282No Hit
CCCCCCCACTATCCCTATAAAAGTTTTCGCGGGACCTAGAAAGCTACATA11.282051282051282No Hit
GAACAATAGCGACACCGTCCAGCTGTCTAAGAACAAGAGTGTGTGGGCCA11.282051282051282No Hit
GTGCTCTTCCGATCTGCAGGGGGGGTGCTTCATACGGGTACTACAACTTA11.282051282051282No Hit
ATCAAGCCGGGTGGGGGGGCGAACGAGGGCTGAGGGGTGCCGCGGGAGCC11.282051282051282No Hit
GGTTAACTAACGGACACTAGGTAGTATTTAGGCTTGGGAGATGGTCCTCC11.282051282051282No Hit
CATACAATCTCACCTCGCAGACGTGTGCTCTAAATATATCCGAAGGGAGA11.282051282051282No Hit
GACCTACATCTACGGAGTTGGGCGTACCACCTCCAAGGAGATCCTTGCGG11.282051282051282No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTGAAAACA11.282051282051282No Hit
CCAGCATCCATGCATGGCCGGATTTTCCGGATCTGTGCTTTGCGCTGGGC11.282051282051282No Hit
TCCGATCTCCCTTGGGAGACGTGTGCTCTTCCTATCTCCCTTGGGAGACG11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ACAACGGAAGAGCACACCACACCCAAACGACAACCGAACAGCACACCTCA11.282051282051282No Hit
CTTGGGAGCCGTGTGCTCCTGCGAGCTCGCGGGGGCGACGGGTGCGGTGG11.282051282051282No Hit
ACTATACGCTATTGGAGCGGGAATTTACCGCGGCTGCTGGCACCAGACTT11.282051282051282No Hit
CTGCTCTTCGCGTATTTTATTTATTTTTTTATTTTTTTTTATTTTTTTTT11.282051282051282No Hit
GGGGGCGGGCGGGGTGCCGCGGGGGCCGTGGGACGGGGTCGGTGACACAG11.282051282051282No Hit
CTATTAGTCATTACGTCGTGAGGTGAAGACGGATGTAAGCAGATCGGAGG11.282051282051282No Hit
CCCGGGAGACGTGTGCTCTTCCGATCGTCCCCGGGAGACGGGTCATCGTC11.282051282051282No Hit
CTTCCGATCTGAGGGGGGCGCGGTCGATCGGCACCGCCTTGCGCCGTCGG11.282051282051282No Hit
GCACTAAACCCCGGAAAGGGTCTAACACCTAGCACTCATCGTTTACGGCG11.282051282051282No Hit
ACCAAGTGGTGCTAATCCAAAAAAAAAAAAAAAATAAATAAAATAAATAA11.282051282051282No Hit
GAACCGAATACCAAACGTCTCACGGTCAAGAACGGAAGAACAAACGTATA11.282051282051282No Hit
GATCGGAAGAGCACACGTCTCCCGGACGAGATCGGAAGAGCACACGTCTC11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GCCCTAATGAGAGGGGTTGAGGCCGGCTGGAAATGGGGGGATATTCTGTG11.282051282051282No Hit
AGGGAGACGTGTGCTCTTCCGATCTCTGTCTCTTATACACATCTGACGCT11.282051282051282Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTTCCGATCTATCAAGGGAAACCACAAGTAAACCGCGCCCGAAACTAAAA11.282051282051282No Hit
ATATAAAAGTGACCTCTTACTATCACACCTGCTAAGACTAAAGCTCATCA11.282051282051282No Hit
AATATGGGGGGTATAGTCCAAGGAGAAGCCACAACATAAAGATAACCTAC11.282051282051282No Hit
CGAATCTGAAGATCACACAAATAGGAAGAGCACACGTATCGGAAGAGCAC11.282051282051282No Hit
GCCTTGTGGTTAGGGCCTGTGGTACTTTGATGGGGTGTATTTGTGAGTTA11.282051282051282No Hit
ACGCAGGGGTGCTCTTCCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
CTTCTCTCTGGTCTCTCTCTTCTCTCTCTTCTCTCTCTTGTCTCGCGGGC11.282051282051282No Hit
AGCCTCAAGTGATGATTATTGGAGGTAGAGCACTGTTTGGACTAGGGGCC11.282051282051282No Hit
CCCTTGGCGTGGCGTGCGGCGGCGCAGTGGCGTGCCATGGCGCCCGCCGC11.282051282051282No Hit
GCCGAAGACCTGGTGGCGTGGGCCGGAGGGGCGGCCGGCCGCCCGGCGGG11.282051282051282No Hit
GGCCATCGGGGATTGGGAAAATCTCTTACGGTCACCACGAATGCGGCTCC11.282051282051282No Hit
GGAGAGCACTTTAAATCCTTTAACAAGGTCCATTTGGAGGGCAAGGCAGG11.282051282051282No Hit
CTCAACTTCCGATCTCTACAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
TGGCGGGGGGGGGCGGGGGGGGGGGCGGGGGGCGCGGGGGGGGGGGGGGG11.282051282051282No Hit
CAAATTTACACTTAATTAGGCATTCAGTGTCACTATGGGATGATTAAAAC11.282051282051282No Hit
CTTCTGGACCCCGCGCACACCGGCCTCGGCTGCTGCCGACATGGACCGCC11.282051282051282No Hit
CTGCCGGTCAGTGTGCAGCTCGCTGACATGCGTTCGTACCGCCTGGAAGA11.282051282051282No Hit
CGTGTGAACCACCCCCTTGGGCCGGCCGGTTGTCCCAGAAGTGTAGGCAA11.282051282051282No Hit
AACCCCAACAAGCTCGGGAAAGTAGTGACCAAAAATAACAATAAAGGACT11.282051282051282No Hit
GGGAGAAAGAAAGGACCCGTACCGCAAACCGACACAGGTCGTCGAGATGA11.282051282051282No Hit
CACGTCTCCCAGTTGAGATCGGAAGAGCACACGTCTCCCTGTAAAGATCG11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
AAACAGATCACCGGCGGATCCTCAAACGAAAAAAAAAAAAAAAAAAAAAA11.282051282051282No Hit
TTGCCGGCGAGGGCGGGGCCGCGGGGGTGGCGGGCGGGCGGGGGGGAGGG11.282051282051282No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGCGTGGGGGGGGGG11.282051282051282No Hit
CATTTGCGCACTGGCGAAGCAGCGAGCAGCGTCGGCTCTCAGCGGCGCTT11.282051282051282No Hit
ATGACAACTAAAAAACACAATTGAACCACCTATAAGAAGACGCATATTCT11.282051282051282No Hit
GCGCCTGGCCGCAGCGAACTAGCTGCGAGAAAGCGCCAAACCCCCTGCCG11.282051282051282No Hit
GGGGGGGGGGGTGGGGGGGGGGGGGGACGGGGGGGGCGGGGGGGGGGGGG11.282051282051282No Hit
CACGTCTCCCGAGCGAGATCGGAAGAGCACACGTCTCCCGAGCGAGATCG11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GAAGAGCACACGTCTCCCGTTGTAGATCGGAAGAGCACCCGTCTCCCGTT11.282051282051282Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGAT50.070.035
ACAAAAA50.070.053
GGATGAT50.070.038
AAACCAA50.070.061
GTCACTA50.070.029
GGCATTC50.070.019
CCAACAT50.070.064
GTGTCAC50.070.027
AAATTTA50.070.02
ACAGACA50.070.049
GAAACCA50.070.060
ATGATTA50.070.040
ATTAGGC50.070.015
AAACAGA50.070.047
AATTTAC50.070.03
TAAAACA50.070.045
TTATTAT50.070.070
TGATTAA50.070.041
ACACTTA50.070.08
TTACACT50.070.06