FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s89.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s89.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1633815
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78620.4812050323935084No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA20210.12369821552623766Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC19440.11898531963533204No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC17760.1087026376915379Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC17300.10588714144502286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGTAC556.6919347E-638.1892973
ATCTTTT103950.033.333747
TCTTTTT104650.032.942548
GATCTTT108450.032.7261626
CTTTTTT106000.032.5560079
TAGGGGT8550.030.7058894
GGTGTAG11150.026.99749869
GAGCATA2500.026.6198791
AGCATAC2450.025.7208982
CGATCTT163850.025.4641845
AGGGGTG31150.024.8332845
CATACGT2550.024.709214
CAGGGGT11000.022.2757264
GTGTAGA14350.021.70891470
GTGTAGT1957.730705E-1021.5545581
GTACATG1859.889845E-920.8263651
CTAGGGG6400.020.7853223
CTTAATC1205.710963E-520.4206683
ATACGTC4000.020.1264765
GTGTAGG5650.019.8378221
Produced by FastQC (version 0.11.5)