FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3580337
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC63080.17618453234988773No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59420.16596203094848336No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC52950.14789110633998978No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA52760.14736043003773108Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC49900.1393723551721528Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC49150.13727758029481582No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC48110.1343728257982419No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGTA27400.025.42278770
TTTCGGA10100.023.9261531
CTTTTTT60900.023.33299
TCTTTTT61600.023.2950238
ATCTTTT63100.023.0192347
TTCGGAA11200.021.2585122
GATCTTT77050.021.2148826
AGCATAC4350.020.1232
TAGGTGT34350.020.07577169
GAAGGCT152350.020.03524670
TAGCATA4600.019.02005821
ACTTTAG4300.018.732881
GAGCATA5100.018.5412391
GCATAAT4950.018.38221423
TTACACC5150.018.3525074
AAGTCAC6150.018.21409668
CATACGT4450.018.0928364
ACCGTTA6000.017.4996768
CGAAGGC179750.017.17641369
TCTGTAT8600.017.0919927
Produced by FastQC (version 0.11.5)