FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1755677
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39120.22282002896888206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG116450.025.282155
ATCTGTG7550.024.5710957
TCTTTTT41550.024.2607778
ATCTTTT41900.023.8917317
CTTTTTT42200.023.7191839
GATCTTT45850.023.5142826
GATCTGG49300.023.2178066
ATCTGAG9450.021.853247
TTCCGAT331100.021.6901682
CCGATCT326900.021.6551324
ATCTGGG21350.021.4767747
CGATCTT79950.021.3239655
GTCATAT3300.021.223141
TCCGATC340950.021.083483
GATCTGA32000.020.1274246
ATACAGA6300.020.0024096
GATCTGC39050.019.8103266
TATACAG6950.019.6443335
TCATATG4100.019.6426662
TCTGTGC6950.019.6409748
Produced by FastQC (version 0.11.5)