FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1161132
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25060.21582386843184065No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA16450.14167209240637585Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC15000.12918427879000838Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC13650.11755769369890762No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC12050.10377803729464007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAATAG2450.034.28959769
TTTCGGA2700.027.234691
TCTTTTT22850.025.5783588
ATCTTTT23400.025.2762837
CTTTTTT23150.025.246899
AGCATAC1451.7234925E-824.1458642
TTCGGAA3300.023.3410022
ACACAAT1802.8558134E-1023.33597467
GATCTTT30300.022.6418516
TTGTCTC952.782541E-422.10395810
ACGATAG4300.021.97923369
TAATGGA800.002665411221.87747663
GACTCCT29450.021.51347270
GAGCATA1656.767186E-821.2218361
CAATAGT1205.712775E-520.4189870
AACGTGT2750.020.36594268
GACACAA1558.7398985E-720.32488366
GTACGTG1055.449764E-420.009161
CATACGT1751.2628698E-720.0039864
AAACGTG2850.019.65134667
Produced by FastQC (version 0.11.5)