FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1329290
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30430.22891919746631662No Hit
TACTAACACCGTCCGATCCCTAGTCGGCATAGTTTATGGTTAAGACTACG18410.138494986045182No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAA15190.11427152840990303No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA6100.030.41140670
CTAGATT500.00721160127.99933836
CGATCTG85250.027.3055155
TCTAGGC3000.026.8326998
ATCTGTG5450.025.6874667
ATCTGAG8750.025.1994047
GATCTTT30150.024.959836
GATCTGG32500.024.8782066
TCTACTT5350.024.8713872
CTACTTG5150.023.7947963
CTAGTCG7950.023.77213120
CCGATCT247900.023.6675514
TTCCGAT249500.023.620212
ATCTGGG13750.023.417637
ATCTTTT27750.023.2066577
CTCTACT5450.023.1352541
TCTTTTT27500.022.908558
TCTGAGC4800.022.6036328
TCCGATC262950.022.5160183
CGATCTT58400.022.4771675
Produced by FastQC (version 0.11.5)