FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s81.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s81.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1055573
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33940.321531528373689No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA12030.11396653760564167Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC11020.10439827468114475Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA3450.031.4704271
TTCGGAA3750.028.945932
TCTTTTT30950.026.6909738
ATCTTTT32300.026.1172567
CTTTTTT31950.025.8518989
GATATGT2001.8189894E-1224.49789254
GATCTTT38150.023.9474336
ATTACTC750.001830313623.3390673
GTAGGAC800.002663202821.8803753
CTCTAGG2201.546141E-1020.6957261
GTCCATA850.003775828320.602081
TAAGGCA850.0037862120.592324
AGTGCCC1408.252951E-619.9992310
AGACTAT1257.8108285E-519.60481667
CGAGTAA1801.6981176E-719.44922370
ACTATTG1107.464219E-419.095669
TATGACA1107.464219E-419.095666
AGGTGTA5900.018.98771570
GAATAGA34050.018.91800970
CGAATAG33000.018.67125369
Produced by FastQC (version 0.11.5)