FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335004
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26440.19805184104317292No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA3500.026.021011
GAGTGTA8650.024.68537170
ATCTTTT25650.024.5609977
GATCTTT30200.023.6419936
CTTTTTT27000.022.8135819
TTCGGAA4250.021.4226442
GATCTGG35000.020.7996546
TCTTTTT29700.020.7403968
GAACGTA2201.5643309E-1020.68070218
AACGTAT2051.4570105E-920.48669819
ATCTGGG14100.020.354277
GCGTATA1801.7043203E-719.4433949
CGATCTG125600.019.3124315
TCTAGGC3150.018.8885758
TAGCCCG1301.05823776E-418.84513710
GTCCTAC950.007177672318.4359381
CAGGGGT4400.018.2978944
ATCTGTG10200.018.1859727
CGATCTT69450.018.0932085
ATCTGCG12000.018.0830337
Produced by FastQC (version 0.11.5)