FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1962716
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATT39020.19880614413903996No Hit
GTACTATTACTTTGAACAAATTAGAGTGCTTAAAGCAGGCTTCAAATGCC33000.1681343607531604No Hit
GTCTTACGACGGTCCAAGAATTTCACCTCTCGCGTCGTAATACTAATGCC32230.16421122566892002No Hit
TCGTAATACTAATGCCCCCAAACTGCTTCTATTAATCATTACCTCTTGAT30980.15784249988281546No Hit
GTTATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACACACGA29040.14795823746278117No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28890.14719399036844863No Hit
GTATAGTACTAACCACAATTGTAAGTTGTACTACCCGTATGAAGCACAAG28340.1443917510225626No Hit
GTATTACGACGCGAGAGGTGAAATTCTTGGACCGTCGTAAGACTAACTTA26760.13634168162892643No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCT26420.134609388215106No Hit
GGATAATGAAATAAGACCTCTGTTCTGCTTTCATTGGTTTTCAGATCAAG26000.13246949635097488No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAAT25530.13007485545539957No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT24730.12599887095229265No Hit
GTCTTGTACCGACGACAGATCTTTCAAATGTCTGCCCTATCAACTTTTGA24400.12431752734476102No Hit
TCATTATCCCATGCACAGAATATTCAGGCATTTGAAGCCTGCTTTAAGCA23870.12161718761145271No Hit
GTACAAGACCATACGATCTGCATGTTATCTAGAGTTCAACCAATATAACG22390.1140766162807049No Hit
GCTTAAAGCAGGCTTCAAATGCCTGAATATTCTGTGCATGGGATAATGAA21900.11158007577255191No Hit
GAACTAGGGCGGTATCTGATCGCCTTCGAACCTCTAACTTTCGTTCTTGA19730.1005239678078744No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA12900.023.6067970
CGTAAAT7500.022.39917623
GCGTAAA7750.021.22448322
CGCGTAA7750.020.772921
GCGCGTA8100.019.87530720
TAGGGGT3750.019.6017764
ACGGTGT16100.019.5669969
GTCCTAG12600.019.1791531
CTAGGGT1651.5929418E-619.092644
AGAAACG12200.018.93614267
ATATAAG10100.018.7183842
TACTATA10150.018.6261752
GATCTGG34150.018.4482846
ACGGTCC12900.018.1776289
CTAGGAC16500.018.0333163
ACTATAC10100.018.0228123
ATACCTT10100.018.0200586
GGTCCAA12500.017.9193411
CTTTTTT27350.017.9152479
ACCGGTA9100.017.69120615
Produced by FastQC (version 0.11.5)