FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1306955
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26890.2057454158712427No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA13350.1021458275151019Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATTAG657.954229E-426.92637670
TCTTTTT20200.023.5636358
ATCTTTT20750.023.4459637
CTTTTTT20350.023.2179629
TAAACGC800.002668407321.87349318
GATCTTT26750.020.934746
CGGTGTA6650.020.5288370
TATAGAC2051.4497346E-920.4942342
CTATAGG2252.1100277E-1020.2339921
GAATTAG33300.019.86729870
TTTCGGA3200.019.6989561
TAGATAC1601.186143E-619.6884045
ACACTAA900.00527584319.44533756
CTAATAG1651.5892947E-619.096173
TAGGGGT5350.018.9757394
AGTACGT1301.0583364E-418.84485616
AATTAGC950.007198075318.4268342
CGAATTA37300.018.39437769
TAGTATT1150.001006772218.2645034
CATACGT1351.4092385E-418.1538452
Produced by FastQC (version 0.11.5)