FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3366929
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC72380.21497334811633986No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA68740.20416230933292626Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65570.19474720138143692No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC64310.1910049187256399Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC64170.19058910954166244No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC59560.17689710712640508No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC58310.17318452512660648No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA5100.028.8462491
AGCATAC2950.024.926742
TCTTTTT40650.023.6770468
AGGTGTA19650.023.51530370
GAGCATA3150.023.3517231
CATACGT3450.023.3371564
CTTTTTT41600.023.2204749
GAAGGCT116600.022.84679470
GGTATAC8550.022.10822964
ATCTTTT43800.021.7351687
TGGTATA5100.020.59038563
TAGGTGT23250.020.3259169
CTTAGGT38550.019.7957270
GATATGT6800.019.5573854
TAGGGGT7600.019.3452764
CGAAGGC139750.019.31264569
CGAGTAA3100.019.19669370
ACATGTG11550.018.79095669
ACGAAGG148750.018.21420768
TACTAGT1351.4084863E-418.1565152
Produced by FastQC (version 0.11.5)