FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1847872
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC57750.31252164652097114No Hit
CGTCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCAC45650.24704092058324387No Hit
CTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGC44480.24070931319918262No Hit
TCCGAGACGTGTGCTCTTCCGAGACGTGTGCTCTTCCGAGACGTGTGCTC42760.23140130918158833No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA41140.22263446818827276Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC39320.21278530114639976Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37900.20510078620164165No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4150.032.0660321
TAAACGT1001.26110535E-831.50047566
TGTGTCA1900.029.4733310
GAGCATA1403.929017E-1027.5153061
TTCGGAA4950.026.8800052
TTAGGTC2050.025.61014251
TAGGTCC2050.025.61014252
GATACTC59700.024.44760770
ACTTTTG1151.4984544E-624.34819269
TTGTGTA9550.024.18884770
CGACTTA2200.023.86205959
GTATAAT901.9292925E-423.33368765
TACAGGA3500.023.0096872
GTGTCAA2450.022.8568711
TCTTTTT23950.022.6504738
CTTTTTT24600.022.4787989
AATCGTT3000.022.16520117
ACTGTGT2700.022.0361778
AGCATAC1755.2332325E-922.0092662
TTAGTGT1755.2405085E-922.0062873
Produced by FastQC (version 0.11.5)