FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4413637
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG85640.19403498747178347No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA45110.10220595848729744Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTTTTT70850.021.8849148
CTTTTTT71600.021.7527039
ATCTTTT73300.021.6789267
AGCATAC4150.021.0928522
GATCTTT93700.019.6114886
TTTCGGA11500.019.1875271
TCTAGGC8900.018.8769668
GATATGC126150.018.5910170
GTGTGTA27100.017.43732670
ATCTGGG50750.017.0351857
CATACGT5150.016.99334
TTCGGAA15150.016.640382
GATCTGG145850.016.5109796
CTAGGCC5800.016.2930369
TCCTACA7850.016.0574382
GCAGTGT46700.015.74047570
TCTAGGG23100.015.6123642
CGATATG150800.015.48263369
GTCTAGG10200.015.4521411
ATCTGGT36850.014.9124167
Produced by FastQC (version 0.11.5)