FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1455633
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34120.23439974224272192No Hit
CGTTGAGACAGTGCCCAGATCGTTACGCCTTTCGTGCGGGTCGGAACTTA19140.13148918717836158No Hit
GAATAGGCCCAGGCGACTGTTTAGCAAAAACACAGGTCTCTGCAAAACCG16190.11122308988598087No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA15760.10826904858573555Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCCTAAGGTGAGCGAGAGAACTCTCGTTAAGGAACTCGGCAAAATGACC15490.10641418544372104No Hit
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC14940.10263576052480261Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATATGT2350.023.82818654
CGTCAAT5150.023.7848123
TCTTTTT20900.023.6116648
CTTTTTT21550.023.0610949
ATCTTTT21750.023.0115287
GTCAATT5500.022.9075524
CAGTCTT3250.022.61386342
TATAGCG1553.5051926E-822.5837825
AGTCTTG3450.022.3173443
ATATGTC1102.9248937E-522.27199455
CGACTAG1604.9059963E-821.87803864
TCTAGGC3200.021.8742798
AGTTTCA5450.021.83339537
TCCTTTG5200.021.53701230
GAGTTTC5550.021.4436
TTCCTTT5250.021.331929
TTGAGTT5750.021.30291634
TCATAAC1656.793016E-821.21506763
TGAGTTT5800.021.11926835
AGACTAT1003.9306373E-421.00291667
Produced by FastQC (version 0.11.5)