FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1415550
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40600.28681431245805517No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTGTA5350.035.33040270
CGATCTG79650.028.4332895
ATCTGTG6950.028.2010827
GATCTGG36150.025.5607476
TTCCGAT202000.025.2683072
GATCTTT27600.024.8556376
ATAGTGT7750.024.84190869
TCTTTTT25200.024.721918
ACTATAC851.3077719E-424.7099363
ATCTTTT26000.024.6343067
CCGATCT205450.024.483564
CTTTTTT25600.024.4723479
TCCGATC209850.024.3213583
TCTAGGC2900.024.1376278
ATCTGGG15700.023.8532037
GTGTATA750.001827353723.3462351
GTCTAGG1352.3030407E-723.3462331
TATACTA750.00183224423.3355125
ATCTGAG7750.023.031977
CTTCCGA225300.022.7711261
Produced by FastQC (version 0.11.5)