FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1346316
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG38190.2836629736258055No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGTA10700.029.11514570
CGATCTG88650.026.45315
GATCTGG36500.026.0819726
ATCTTTT34350.025.2690727
TCTTTTT33800.025.2660588
GATCTTT37550.025.0730236
ATCTGAG8650.024.277257
CTTTTTT35700.023.9222539
ATCTGTG7250.023.6549727
ATCTGGG16050.023.3331367
TTCCGAT250600.023.0644592
TCTGAGG4250.023.0586268
CCGATCT251700.022.6804284
TCCGATC256450.022.5358123
GTATAGT2350.022.3535211
CGATCTT65250.022.046615
TACACAG2600.021.539085
GAGGTGT15200.020.72581969
GTACATA850.003778249520.6003021
CTTCCGA282200.020.5382541
Produced by FastQC (version 0.11.5)