FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1255512
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG24490.19505986402360154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA3700.032.1859131
AGCATAC1154.883441E-827.4038892
GAGTGTA9450.026.66829170
GTACTGA1451.7162165E-824.1557581
CTAGTAG1052.0354655E-523.3412633
CATACGT1553.504283E-822.583824
TTCTATA1154.105335E-521.3141352
ATCTTTT29650.021.1302937
TCTGAGC5500.021.0004438
TTCGGAA5400.020.750272
CTTTTTT29600.020.6930059
AGAGTGT12250.020.57350269
GATCTTT35250.020.3550386
TCTTTTT30750.020.260598
GGGACAT1055.46617E-420.000426
ATCTGGG12900.019.5352957
ATCTGAG11350.019.1193457
CTAGCGC1107.4765855E-419.0913129
TAGGGGT4500.018.6692894
CTCTAGG1902.9843432E-718.4346581
Produced by FastQC (version 0.11.5)