FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1566605
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34530.22041293114728983No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA19260.12294100938015647Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC17290.11036604632309996Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4500.029.5754721
TCTTTTT47850.026.6249548
ATCTTTT50050.025.735177
CTTTTTT50250.025.3533139
AGCATAC1601.8571882E-924.0717962
GATCTTT59250.023.0985956
TTCGGAA5650.022.929212
GAGCATA1854.0017767E-1022.7180041
TAATACT952.7795695E-422.1081564
TATGCTT1755.2496034E-922.0028785
CAGGGGT6150.021.6288474
TCTAGGC4050.021.6050078
CGGTGTA14950.021.30645870
CATTAGC3050.020.6571246
TAAGAGT1709.319592E-820.58961768
AGGGGTG16150.020.1574365
TAGGGGT4900.020.0026194
GTCTAGG3750.019.6132071
TAGTACA900.005273711419.4469914
TACATTA3250.019.3871524
Produced by FastQC (version 0.11.5)