FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1630879
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37410.22938550315504705No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT44650.027.7489057
GATCTTT49750.027.1555296
TCTTTTT44950.027.0965238
CTTTTTT45850.026.411979
AGGTGTA15750.025.55688970
TTTCGGA5850.023.3481521
TAGGTGT18700.021.71236269
TCTGAGG3400.021.6174498
ATCTGTG11000.020.6816277
CGATCTT99450.020.2737925
CAGGGGT6350.019.845384
ATCTGAG10550.018.9097797
GCCTAGG3550.018.7442911
TTAGGTG21500.018.55910768
TATATAC1000.00967547517.5052073
TACACCG5000.017.5019845
GATCTGG49200.017.144156
GTATTGG3501.8189894E-1217.0107981
TTCGGAA7850.016.9497972
AGGGGTG17400.016.8984685
Produced by FastQC (version 0.11.5)