FastQCFastQC Report
Thu 21 Nov 2019
HW7KNAFXY_n02_hr38s6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7KNAFXY_n02_hr38s6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1876134
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG39570.2109124401561935No Hit
TCTCGGAAGAGCACACGTCTCGGAAGAGCACACGTCTCGGAAGAGCACAC20280.10809462437118031No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGA20210.10772151669337052Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
ATCTAGACGTGTGCTCTTCCGATCTAGACGTGTGCTCTTCCGATCTAGAC19910.1061224837884714Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTT67050.027.8216427
TCTTTTT67950.027.1956088
GATCTTT74300.026.9917936
CTTTTTT68750.026.9300589
AGGTGTA17150.023.26643270
TAGGGGT7150.022.0313854
CGATCTT137850.020.4906525
TATAAGG2603.6379788E-1220.1986682
TAGGTGT19550.020.05212269
CATACGT2302.8376235E-1019.788842
TTTCGGA7200.019.4562761
TAATAGT900.00527335819.4474564
GATCTGG59600.018.4395076
GTGTAAG4200.018.344491
CTAGGGG4400.018.2987773
TCTGTGC10000.018.1994258
GTATTGG5250.018.0109521
AGGTATA2551.1295924E-917.84257363
TCTGTAT4650.017.31128127
AGGGGTG23700.017.2793185
Produced by FastQC (version 0.11.5)